This study presents a novel mutant resource representing a whole genome sequenced EMS mutagenized oilseed rape population (Harloff et al. 2012). The platform will serve as an important resource in aiding reverse genetics based studies in oilseed rape in a cost and time-effective manner. Using a whole genome NGS approach to detect and screen EMS induced mutations, the resource enables the selection of EMS induced mutants that possess putative functional mutations within any candidate gene/genomic region of interest.
1. As a first step, the Express617 reference genome assembly and the supplemented GFF file must be downloaded from https://doi.org/10.5281/zenodo.3524259.
2. By using BLAST queries, genomic regions of interest within this reference assembly with respect to the chromosome and their exact locations can be identified.
3. Our dataset can be filtered based on the “CHROMOSOME”, “START” and “END” information. By screening the dataset, mutations within genomic regions of interest can be identified in the respective 4x pools.